Erroneously low creatine kinase activity measurements with use of the Calbiochem CPK-MB kit with the Beckman TR Enzyme Analyzer.
نویسندگان
چکیده
and biosynthesis. Clin. Chem. 23, 5 (1977). Review. 2. Brown, J. R., Structure of bovine serum albumin. Fed. Proc. Fed. Am. Soc. Exp. Biol. 34, 591 (1975). Abstract 2105. 3. Weijers, R. N. M., Thesis, University of Utrecht, The Netherlands, 1971. 4. Weijers, R., Hagel, P., Das, B. ., and van der Meer, C., Tryptic peptides from rabbit and bovine albumin enhancing the effect of bradykinin. Biochim. Biophys. Acta 279,331 (1972). 5. Segel, I. H., Biochemical Calculations, John Wiley & Sons, Inc., New York, N. Y., 1968, pp 139-149, 347-349. 6. Shearer, W. I., Bradshaw, R. A., Gurd, F. R. N., and Peters, T., Jr., The amino acid sequence and copper (11)-binding properties of peptide (1-24) of bovine serum albumin. J. Biol. Chem. 242, 5451 (1967). 7. Meloun, B., Mor#{225}vek, L,, and Kostka, V., Complete amino acid sequence of human serum albumin. FEBS Lett. 58, 134 (1975).
منابع مشابه
Evaluation of three current methods for the determination of creatine kinase-MB catalytic activity.
Three current methods for the determination of the creatine kinase-MB activity were evaluated: electrophoresis (Beckman Inst.), ion-exchange MB-Zyme (J.T. Baker) and ion-exchange on aca (Du Pont). Eighty patients were selected out of three groups: acute myocardial infarction, heart and vascular surgery patients and a number of noncardiac cases. The precision of the ion-exchange methods was comp...
متن کاملComparisonof Per - cent of ExpectedValue and Percent Recoveryfor Serum Folate Radioassay
The LKB 8600 Reaction Rate Analyzer sold in the United States, is equipped with a “delay switch,” whereas the same instrument sold in Canada measures time rate reactions immediately after the addition of the initiating reagent, without provision for delay. Therefore, “lag-phases” of any enzyme reaction are recorded with the Canadian version. One of the basics of zero-order kinetic assays is the...
متن کاملSerial serum creatine phosphokinase MB isoenzyme activity after myocardial infarction. Studies in the baboon and man.
Serum CPK-MB iosenzyme activity was measured serially after mercury embolization of the left circumflex coronary artery in five baboons and after clinical acute myocardial infarction (AMI) in 20 patients. The calculated amount of enzyme released into the baboons' circulation (CPK-MBR) correlated well (r = 0.991) with the amount of MB isoenzyme depleted from the myocardium (CPK-MBD) when a previ...
متن کاملNew manual and automated method for determining activity of creatine kinase isoenzyme MB, by use of dithiothreitol: clinical applications.
The method is based on the selective activating capacity of dithiothreitol on creatine kinase isoenzyme MB, after isoenzyme MM is activated by glutathione. Isolated isoenzymes MM and MB of human and canine origin were assayed individually and in mixtures of known activities. When glutathione was present in the assay medium the activity of each isoenzyme could be measured individually, but gluta...
متن کاملCreatine kinase kit formulations compared for suitability in development of creatine kinase isoenzymes on cellulose acetate.
To the Editor: The last step in electrophoretic procedures for creatine kinase (CK; EC 2.7.3.2) isoenzyme separation on cellulose acetate is to incubate the plate with a reagent that will develop either fluorescence or color for each isoenzyme. In our laboratory we routinely use fluorescence to detect the isoenzymes. The reagent used must be highly sensitive, so that low proportions of the isoe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 23 7 شماره
صفحات -
تاریخ انتشار 1977